Translational_Unit

Part:BBa_K2123201:Design

Designed by: Maria Clara Tavares Astolfi   Group: iGEM16_UFAM-UEA_Brazil   (2016-10-10)


Strong RBS + MerB (Organomercurial Lyase) + Strong RBS + MerA (Mercuric Reductase) + B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 458
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 56
    Illegal NgoMIV site found at 630
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 49


Design Notes

PCR primer


Source

pO26-CRL

References